Promoter Region Analysis of STAMP1/STEAP2 Gene-Silencing of STAMP1/STEAP2 Gene Triggers P53 Upregulation

Prostate cancer is the most commonly diagnosed and second leading cause of mortality in men Western World. In initial stages, prostate dependent on androgens for growth which basis androgen ablation therapy. The effects are mediated by Androgen Receptor (AR). Therefore, studies focus identification AR-regulated genes that also highly expressed prostate. STAMP family STAMP1/STEAP2 STAMP2/STEAP4 have only receptor-positive cells, role AR gene expression an important question. STEAP (Six Transmembrane Epithelial Antigen Prostate) first characterized enriched six transmembrane gene, metastatic samples, it tempting to speculate STAMP/STEAP may be involved similar functions with a both normal biology pathophysiology Using siRNA technology LNCaP cells expressing per se, apoptosis panel including pro-apoptotic and/or apoptotic molecules was assayed RT-PCR, By this research project, prostate-specific its regulatory p53- caspase-related pathways were characterized.